报告人：Artem Pliss（University at Buffalo）

时  间：2024年11月11日上午10：00

地  点：深圳大学致原楼1214会议室

邀请人：屈军乐教授

摘要：Prostate  tumors commonly exhibit pronounced accumulations of saturated lipids  and cholesterol due to upregulated lipogenesis and intracellular uptake  of circulating lipoproteins. Furthermore, the lipids deposits fuel the  progression of once benign lesions to increasingly aggressive and  metastatic tumors. To mitigate the spread of lethal tumors, a  suppression of a key lipogenic enzyme, fatty acid synthase (FASN), has  been recently proposed, although the first trials did not produce any  convincing results. To unravel a correlation between lipids metabolism  and the behavior of prostate cancer cells, we have applied an innovative  microlipidomics approach, based on vibrational Raman spectroscopy, and  applied it for the analysis of major organelles involved in lipid  synthesis, metabolism and storage: endoplasmic reticulum, Golgi  apparatus and lipid droplets in prostate cancer cells.  

We  found that lipidomic profiles in these organelles reflect a dynamic  balance between de novo lipogenesis and the uptake of cholesterol and  fatty acids from the medium.  Strikingly, an inhibition of FASN is  accompanied by dramatic activation of lipids uptake, leading to even  more pronounced accumulation of lipids in the treated cells.  Furthermore, the excessive accumulation of saturated lipids and  cholesterol correlated with the higher migration of the treated cells  and presumably higher metastatic activity. Thus, our data indicate that  inhibition of FASN could be beneficial for prostate cancer treatment  only when circulating in bloodstream lipoproteins providing pool of  lipids are depleted by diet and/or exercise.    

简介：Artem’s  multidisciplinary research is on the interface of cell science,  biophotonics and bioengineering. He investigates cellular physiology  using quantitative optical approaches including conventional, confocal,  and multiphoton fluorescence microscopy, Fluorescence Lifetime Imaging  (FLIM), Second Harmonic Generation (SHG) imaging and micro-Raman  spectroscopy (BCAbox). This research is focused on the molecular  environment in vital cellular organelles and its transformations  throughout cellular growth, disease progression or drug-cell  interactions. Artem is also very interested in development and  applications of advanced biophotonics and nanophotonics technology for  modern biomedical research.